Abstract
An expressed sequence tag-based microarray was used to profile genome expression underlying light control of Arabidopsis development. Qualitatively similar gene expression profiles were observed among seedlings grown in different light qualities, including far-red, red, and blue light, which are mediated primarily by phytochrome A, phytochrome B, and the cryptochromes, respectively. Furthermore, light/dark transitions also triggered similar differential genome expression profiles. Most light treatments also resulted in distinct expression profiles in small fractions of the expressed sequence tags examined. The similarly regulated genes in all light conditions were estimated to account for approximately one-third of the genome, with three-fifths upregulated and two-fifths downregulated by light. Analysis of those light-regulated genes revealed more than 26 cellular pathways that are regulated coordinately by light. Thus, light controls Arabidopsis development through coordinately regulating metabolic and regulatory pathways.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Arabidopsis / genetics
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Arabidopsis / growth & development*
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Arabidopsis / radiation effects
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Arabidopsis Proteins
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Cryptochromes
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Drosophila Proteins*
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Expressed Sequence Tags
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Eye Proteins*
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Flavoproteins / genetics
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Flavoproteins / radiation effects
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Gene Expression Regulation, Developmental / radiation effects
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Gene Expression Regulation, Plant / radiation effects
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Genome, Plant*
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Light
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Oligonucleotide Array Sequence Analysis
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Photoreceptor Cells / radiation effects
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Photoreceptor Cells, Invertebrate*
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Phytochrome / genetics
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Phytochrome / radiation effects
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Phytochrome A
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Phytochrome B
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Plant Proteins / genetics*
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Plant Proteins / radiation effects
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Plant Shoots / genetics
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Plant Shoots / growth & development
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Plant Shoots / radiation effects
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Receptors, G-Protein-Coupled
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Transcription Factors*
Substances
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Arabidopsis Proteins
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CRY1 protein, Arabidopsis
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Cryptochromes
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Drosophila Proteins
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Eye Proteins
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Flavoproteins
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PHYA protein, Arabidopsis
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PHYB protein, Arabidopsis
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Phytochrome A
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Plant Proteins
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Receptors, G-Protein-Coupled
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Transcription Factors
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cry protein, Drosophila
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Phytochrome
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Phytochrome B