Abstract
The hematopoietic cell-specific adaptor protein, SLP-76, is critical for T cell development and mature T cell receptor (TCR) signaling; however, the structural requirements of SLP-76 for mediating thymopoiesis and mature T cell function remain largely unknown. In this study, transgenic mice were generated to examine the requirements for specific domains of SLP-76 in thymocytes and peripheral T cells in vivo. Examination of mice expressing various mutants of SLP-76 on the null background demonstrates a differential requirement for specific domains of SLP-76 in thymocytes and T cells and provides new insight into the molecular mechanisms underlying SLP-76 function.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Amino Acid Motifs
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Amino Acid Substitution
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Animals
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Binding Sites
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CD3 Complex / immunology
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Calcium Signaling
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Carrier Proteins / physiology
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Cell Differentiation
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Clonal Deletion / physiology
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Immunophenotyping
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Membrane Proteins*
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Transgenic
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Mutation, Missense
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Phosphoproteins / chemistry
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Phosphoproteins / deficiency
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Phosphoproteins / genetics
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Phosphoproteins / physiology*
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Protein Structure, Tertiary
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Receptors, Antigen, T-Cell / immunology
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / physiology
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Sequence Deletion
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Signal Transduction / physiology
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Spleen / immunology
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Structure-Activity Relationship
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T-Lymphocytes / cytology*
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T-Lymphocytes / immunology
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Thymus Gland / immunology
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src Homology Domains
Substances
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Adaptor Proteins, Signal Transducing
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CD3 Complex
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Carrier Proteins
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Lat protein, mouse
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Membrane Proteins
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Phosphoproteins
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Receptors, Antigen, T-Cell
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Recombinant Fusion Proteins
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SLP-76 signal Transducing adaptor proteins