CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation

Immunity. 2001 Dec;15(6):1051-61. doi: 10.1016/s1074-7613(01)00252-7.

Abstract

CD8 serves both as an adhesion molecule for class I MHC molecules and as a coreceptor with the TCR for T cell activation. Here we study the developmental regulation of CD8-mediated binding to noncognate peptide/MHC ligands (i.e., those not bound by the TCR). We show that CD8's ability to bind soluble class I MHC tetramers and to mediate T cell adhesion under shear flow conditions diminishes as double-positive thymocytes mature into CD8(+) T cells. Furthermore, we provide evidence that this decreased CD8 binding results from increased T cell sialylation upon T cell maturation. These data suggest that CD8's ability to interact with class I MHC is not fixed and is developmentally regulated through the T cell's glycosylation state.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 2
  • ATP-Binding Cassette Transporters / genetics
  • Animals
  • CD3 Complex / metabolism
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8 Antigens / immunology
  • CD8 Antigens / metabolism*
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / metabolism
  • Cell Adhesion
  • Cell Differentiation
  • Cellular Senescence
  • Glycosylation
  • H-2 Antigens / immunology
  • H-2 Antigens / metabolism*
  • Histocompatibility Antigen H-2D
  • Ligands
  • Macromolecular Substances
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • N-Acetylneuraminic Acid / metabolism
  • Neuraminidase / pharmacology
  • Ovalbumin / immunology
  • Peptide Fragments / immunology
  • Protein Binding
  • Protein Processing, Post-Translational*
  • Receptors, Antigen, T-Cell, alpha-beta / deficiency
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Rheology
  • Solubility
  • T-Lymphocyte Subsets / cytology*
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism
  • Thymus Gland / cytology
  • Thymus Gland / growth & development

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 2
  • ATP-Binding Cassette Transporters
  • CD3 Complex
  • CD8 Antigens
  • H-2 Antigens
  • H-2Kb protein, mouse
  • Histocompatibility Antigen H-2D
  • Ligands
  • Macromolecular Substances
  • Membrane Glycoproteins
  • Peptide Fragments
  • Receptors, Antigen, T-Cell, alpha-beta
  • TAP1 protein, human
  • Tap1 protein, mouse
  • Ovalbumin
  • Neuraminidase
  • N-Acetylneuraminic Acid