Differential gene expression in neutrophils from patients with generalized aggressive periodontitis

J Periodontal Res. 2001 Dec;36(6):390-7. doi: 10.1034/j.1600-0765.2001.360607.x.

Abstract

Differential gene expression was investigated in neutrophils stimulated with N-formyl-methionyl-leucyl-phenylalanine using RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP-PCR). The cells were isolated from 3 groups of subjects: patients with generalized aggressive periodontitis (Aggressive-P. n = 6), generalized chronic periodontitis (Chronic-P, n = 6) and healthy controls (H, n = 8). Our results show that 37 genes were upregulated. while 27 genes were down-regulated in all Aggressive-P neutrophils by using RAP-PCR with 45 primer pairs. Reverse transcription-PCR analyses revealed that mRNA levels were significantly different (p<0.05) for heat shock transcription factor 4b (HSF4b) gene. Kruppel-like zinc finger transcription factor 9 (Zf9) and muskelin genes. HSF4b was greater in neutrophils from Aggressive-P compared to groups H and Chronic-P. Zf9 and muskelin genes were lower in Aggressive-P compared to the H groups, but no significant difference was noted compared to the Chronic-P group. The control genes, IL-1beta and VEGF genes, were expressed at a significantly higher level in Aggressive-P and Chronic-P than H (p<.01, p<0.05). In conclusion, the RAP-PCR technique used in this study enabled us to identify 3 Aggressive-P related genes, which had not been reported previously. Neutrophil functions in Aggressive-P patients are suggested to be altered by regulatory factors of the immune system including HSF4b (transcription factor), Zf9 (activator of TGF-beta) and muskelin (cellular adhesion).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aggressive Periodontitis / genetics
  • Aggressive Periodontitis / immunology
  • Aggressive Periodontitis / metabolism
  • Case-Control Studies
  • Cell Adhesion Molecules
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics
  • Dual-Specificity Phosphatases
  • Female
  • Gene Expression
  • Heat Shock Transcription Factors
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Intracellular Signaling Peptides and Proteins
  • Kruppel-Like Factor 6
  • Kruppel-Like Transcription Factors
  • Male
  • Mitogen-Activated Protein Kinase Phosphatases
  • Neutrophils / metabolism
  • Periodontitis / genetics*
  • Periodontitis / immunology*
  • Periodontitis / metabolism
  • Phosphofructokinases / biosynthesis
  • Phosphofructokinases / genetics
  • Protein Biosynthesis
  • Protein Tyrosine Phosphatases / biosynthesis
  • Protein Tyrosine Phosphatases / genetics
  • Proteins / genetics
  • Proto-Oncogene Proteins*
  • RNA / analysis
  • Random Amplified Polymorphic DNA Technique
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Trans-Activators / biosynthesis
  • Trans-Activators / genetics
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics

Substances

  • Cell Adhesion Molecules
  • DNA-Binding Proteins
  • GREM1 protein, human
  • HSF4 protein, human
  • Heat Shock Transcription Factors
  • Hsf4 protein, mouse
  • Intercellular Signaling Peptides and Proteins
  • Intracellular Signaling Peptides and Proteins
  • KLF6 protein, human
  • Klf6 protein, mouse
  • Kruppel-Like Factor 6
  • Kruppel-Like Transcription Factors
  • MKLN1 protein, human
  • Mkln1 protein, mouse
  • Proteins
  • Proto-Oncogene Proteins
  • Trans-Activators
  • Transcription Factors
  • RNA
  • Phosphofructokinases
  • DUSP10 protein, human
  • Mitogen-Activated Protein Kinase Phosphatases
  • Dual-Specificity Phosphatases
  • Protein Tyrosine Phosphatases