Objective: To analyze the genotype of the measles virus epidemic strains in Dongguan according to the patients suffered measles in 2000.
Methods: The saliva of 55 patients with acute measles symptoms were collected and detected with RT-PCR. The PCR products of the 5 samples were sequenced and analyzed with the software DNASTAR.
Results: The positive detection rate was 87.3%, whereas the positive rate of anti MV-IgM in serum was only 70.9%. 68.8% of the saliva samples of patients with anti MV-IgM negative results showing MV RNA positive. The 293 bp fragment of N gene and 194 bp fragment of M gene were amplified from the vaccine virus and the specimens. The sequences of the epidemic strains were identical in both M gene and N gene.
Conclusion: These results suggested that the outbreak came from a single MV strain. The epidemic strain was obviously different from the standard strains discovered in other countries of the world (the deviation were 1.4%-11.7%) that might belong to H genotype.