Abstract
The presence of galactose alpha-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the alpha-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Alleles
-
Animals
-
Animals, Genetically Modified*
-
Cell Line
-
Cloning, Organism*
-
Embryo Transfer
-
Female
-
Fetus
-
Fibroblasts
-
Galactosyltransferases / genetics*
-
Gene Targeting*
-
Genetic Vectors
-
Male
-
Mutagenesis, Insertional
-
Nuclear Transfer Techniques
-
Pregnancy
-
Recombination, Genetic
-
Swine
-
Swine, Miniature / embryology
-
Swine, Miniature / genetics*
-
Transfection
Substances
-
Galactosyltransferases
-
N-acetyllactosaminide alpha-1,3-galactosyltransferase