The nucle(ol)ar Tif6p and Efl1p are required for a late cytoplasmic step of ribosome synthesis

B Senger; D L Lafontaine; J S Graindorge; O Gadal; A Camasses; A Sanni; J M Garnier; M Breitenbach; E Hurt; F Fasiolo

doi:10.1016/s1097-2765(01)00403-8

The nucle(ol)ar Tif6p and Efl1p are required for a late cytoplasmic step of ribosome synthesis

Mol Cell. 2001 Dec;8(6):1363-73. doi: 10.1016/s1097-2765(01)00403-8.

Authors

B Senger¹, D L Lafontaine, J S Graindorge, O Gadal, A Camasses, A Sanni, J M Garnier, M Breitenbach, E Hurt, F Fasiolo

Affiliation

¹ UPR no 9002 du CNRS, Institut de Biologie Moleculaire et Cellulaire du CNRS, 15 Rue Rene Descartes, 67084 Strasbourg Cedex, France.

PMID: 11779510
DOI: 10.1016/s1097-2765(01)00403-8

Abstract

Deletion of elongation factor-like 1 (Efl1p), a cytoplasmic GTPase homologous to the ribosomal translocases EF-G/EF-2, results in nucle(ol)ar pre-rRNA processing and pre-60S subunits export defects. Efl1p interacts genetically with Tif6p, a nucle(ol)ar protein stably associated with pre-60S subunits and required for their synthesis and nuclear exit. In the absence of Efl1p, 50% of Tif6p is relocated to the cytoplasm. In vitro, the GTPase activity of Efl1p is stimulated by 60S, and Efl1p promotes the dissociation of Tif6p-60S complexes. We propose that Tif6p binds to the pre-60S subunits in the nucle(ol)us and escorts them to the cytoplasm where the GTPase activity of Efl1p triggers a late structural rearrangement, which facilitates the release of Tif6p and its recycling to the nucle(ol)us.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Transport
Cell Division
Cell Nucleolus / metabolism*
Cell Nucleus / metabolism*
Conserved Sequence
Cytoplasm / enzymology
Cytoplasm / metabolism*
Enzyme Activation
GTP Phosphohydrolases / chemistry
GTP Phosphohydrolases / genetics
GTP Phosphohydrolases / metabolism*
Gene Deletion
Genes, Reporter / genetics
Molecular Weight
Phenotype
Protein Subunits
RNA Precursors / chemistry
RNA Precursors / genetics
RNA Precursors / metabolism
RNA Processing, Post-Transcriptional*
RNA, Fungal / chemistry
RNA, Fungal / genetics
RNA, Fungal / metabolism
RNA, Ribosomal / chemistry
RNA, Ribosomal / genetics
RNA, Ribosomal / metabolism
Ribosomes / chemistry
Ribosomes / metabolism*
Saccharomyces cerevisiae / cytology
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / growth & development
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / chemistry
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism

Substances

Protein Subunits
RNA Precursors
RNA, Fungal
RNA, Ribosomal
Saccharomyces cerevisiae Proteins
GTP Phosphohydrolases