Objective: To investigate the effects of hexamethylene bisacetamide (HMBA) on the in vitro adhesion, motility and invasion of human mucoepidermoid carcinoma cell line MEC-1.
Methods: MEC-1 cells were induced with 2 mmol/L of HMBA in vitro, the adhesion of MEC-1 cells to the reconstituted basement membrane Matrigel was investigated with MTT colorimetric assay; the motility and invasion of MEC-1 cells were assayed using biopore membrane Millicell-PCF inserts culture system.
Results: The adhesion of MEC-1 cells treated with HMBA for 30 min or 90 min reduced by 26.47% and 31.13%, respectively; the motility and invasion of MEC-1 cells treated with HMBA reduced by 63.49% and 67.65%, respectively.
Conclusions: HMBA has anti-invasion effects on human mucoepidermoid carcinoma cells in vitro.