Abstract
Metastatic breast cancer requires systemic treatment. We have developed a systemic gene therapy approach for breast cancer, consisting of a nonviral gene delivery system (SN) and a proapoptotic gene, bik. The transfection efficiency of SN carrying a reporter gene was 5-10 times higher than the common nonviral agents Fugene-6 and Lipofectamine in the presence of serum. The SN-bik gene complex induced significant apoptosis in four breast cancer cell lines in vitro as well as in orthotopic tumor tissues in nude mice. Systemically administrated SN-bik significantly inhibited the growth and metastasis of human breast cancer cells implanted in nude mice and prolonged the life span of the treated animals. This study demonstrates that SN-bik is a promising approach for further development as a potential therapeutic agent of cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / genetics*
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Apoptosis Regulatory Proteins
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Breast Neoplasms / genetics*
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Breast Neoplasms / pathology
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Breast Neoplasms / therapy*
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Carcinoma, Non-Small-Cell Lung / genetics
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Carcinoma, Non-Small-Cell Lung / pathology
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Carcinoma, Non-Small-Cell Lung / therapy
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Cations
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Cell Division / genetics
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DNA / administration & dosage*
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DNA / genetics
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Female
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Genetic Therapy / methods*
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Green Fluorescent Proteins
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Liposomes / administration & dosage
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Luminescent Proteins / biosynthesis
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Luminescent Proteins / genetics
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Lung Neoplasms / genetics
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Lung Neoplasms / pathology
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Lung Neoplasms / therapy
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Membrane Proteins*
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Mice
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Mice, Nude
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Mitochondrial Proteins
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Protein Biosynthesis
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Proteins / genetics*
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Proteins / physiology
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Transfection
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Tumor Cells, Cultured
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Xenograft Model Antitumor Assays
Substances
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Apoptosis Regulatory Proteins
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BIK protein, human
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Cations
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Liposomes
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Luminescent Proteins
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Membrane Proteins
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Mitochondrial Proteins
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Proteins
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Green Fluorescent Proteins
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DNA