Identification of foreign gene sequences by transcript filtering against the human genome

Nat Genet. 2002 Feb;30(2):141-2. doi: 10.1038/ng818. Epub 2002 Jan 14.

Abstract

We have developed a computational subtraction approach to detect microbial causes for putative infectious diseases by filtering a set of human tissue-derived sequences against the human genome. We demonstrate the potential of this method by identifying sequences from known pathogens in established expressed-sequence tag libraries.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Databases, Genetic
  • Expressed Sequence Tags
  • Gene Library
  • Genetics, Microbial*
  • Genome, Human*
  • Genome, Viral
  • Humans
  • Sequence Analysis, DNA
  • Viruses / genetics
  • Viruses / pathogenicity

Substances

  • DNA, Viral