Abstract
In this study, we report the exact localization and substitutional characterization of a B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen. A set of deletion variants containing preS2 sequences of different length was generated on the basis of frCP as a carrier. It was found after Western blot analysis that three monoclonal antibodies (MAbs) (2-11B1, 3-11C2, HB.OT10) recognized the linear preS2 sequence within the amino acid (aa) stretch 3-WNSTTFHQTLQDP-13. The importance of each aa residue of the epitope was proved by comparison of antibody binding to alanine-substituted peptides in both free-peptide and Pepscan variants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / immunology
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Blotting, Western
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Epitope Mapping*
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Epitopes, B-Lymphocyte / analysis*
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Epitopes, B-Lymphocyte / genetics
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Epitopes, B-Lymphocyte / immunology
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Hepatitis B Surface Antigens / analysis*
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Hepatitis B Surface Antigens / genetics
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Hepatitis B Surface Antigens / immunology
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Mice
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Molecular Sequence Data
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Protein Precursors / analysis*
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Protein Precursors / genetics
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Protein Precursors / immunology
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology
Substances
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Antibodies, Monoclonal
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Epitopes, B-Lymphocyte
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Hepatitis B Surface Antigens
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Protein Precursors
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Viral Envelope Proteins
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presurface protein 2, hepatitis B surface antigen