Abstract
To explore the possibility of expressing membrane-anchored exodomains of heterologous surface antigens in Giardia, a chimeric construct containing the Toxoplasma gondii SAG1 gene was made. The Giardia system is shown here to provide a means of generating correctly folded chimeric surface proteins in a native and unmodified form.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Protozoan / blood
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Antibodies, Protozoan / immunology
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Antigens, Protozoan / chemistry
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Antigens, Protozoan / genetics
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Antigens, Protozoan / immunology
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Antigens, Surface / chemistry
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Antigens, Surface / genetics
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Antigens, Surface / immunology
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Cell Membrane
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Gene Expression
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Genetic Vectors*
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Giardia*
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Humans
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Protein Conformation
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Protein Processing, Post-Translational
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Protozoan Proteins / chemistry
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Protozoan Proteins / genetics
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Protozoan Proteins / immunology*
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Toxoplasma / genetics
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Toxoplasma / immunology*
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Toxoplasmosis / blood
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Toxoplasmosis / immunology
Substances
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Antibodies, Protozoan
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Antigens, Protozoan
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Antigens, Surface
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Protozoan Proteins
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Recombinant Fusion Proteins
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SAG1 antigen, Toxoplasma
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variant surface protein H7, Giardia lamblia