Marked increase in number of dendritic cells in autoimmune-prone (NZW x BXSB)F1 mice with age

Yashusi Adachi; Shigeru Taketani; Junko Toki; Kazuya Ikebukuro; Kikuya Sugiura; Haruki Oyaizu; Ryoji Yasumizu; Minoru Tomita; Hiroyuki Kaneda; Yasuo Amoh; Tomoki Ito; Mitsuhiko Okigaki; Muneo Inaba; Susumu Ikehara

doi:10.1634/stemcells.20-1-61

Marked increase in number of dendritic cells in autoimmune-prone (NZW x BXSB)F1 mice with age

Stem Cells. 2002;20(1):61-72. doi: 10.1634/stemcells.20-1-61.

Authors

Yashusi Adachi¹, Shigeru Taketani, Junko Toki, Kazuya Ikebukuro, Kikuya Sugiura, Haruki Oyaizu, Ryoji Yasumizu, Minoru Tomita, Hiroyuki Kaneda, Yasuo Amoh, Tomoki Ito, Mitsuhiko Okigaki, Muneo Inaba, Susumu Ikehara

Affiliation

¹ First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan.

PMID: 11796923
DOI: 10.1634/stemcells.20-1-61

Abstract

Here, we report that the number of CD11c(+)CD3(-) B220(-) cells increases in autoimmune-prone male (NZW x BXSB)F1 (W/BF1) mice with age. The CD11c(+)CD3(-)B220(-) cells from W/BF1 mice show a typical stellate shape and induce the proliferation of T cells. In the CD11c(+)CD3(-)B220(-) cells from W/BF1 mice, CD11b (Mac-1alpha), NK 1.1, and CD95 (Fas) are upregulated in comparison with normal mice, while the expression of CD8alpha, CD117 (c-kit), CD135 (Flk-2/Flt-3), and Sca-1 decreases. There is a significant increase in Flt-3L (FL) mRNA in the bone marrow of W/BF1 mice with age. Moreover, activated hemopoietic cells express high levels of FL. The injection of CD11c(+)CD3(-)B220(-) cells from old W/BF1 mice to young W/BF1 mice transiently induces autoimmune disease (thrombocytopenia). These results suggest that hyperproduction of FL from activated hemopoietic cells induces a dramatic increase in the number of dendritic cells in aged W/BF1 mice, followed by the acceleration of autoimmunity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Marrow / metabolism
CD11 Antigens / biosynthesis
CD4-Positive T-Lymphocytes / metabolism
CD8 Antigens / biosynthesis
Cell Division
Cell Line
Cell Membrane / metabolism
Crosses, Genetic
Dendritic Cells / cytology*
Dendritic Cells / metabolism
Endocytosis
Flow Cytometry
Integrin alphaXbeta2 / biosynthesis
Leukocytes, Mononuclear / metabolism
Lymphocytes / metabolism
Membrane Proteins / biosynthesis
Mice
Phagocytosis
Proto-Oncogene Proteins / biosynthesis
Proto-Oncogene Proteins c-kit / biosynthesis
RNA, Messenger / metabolism
Receptor Protein-Tyrosine Kinases / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Thrombocytopenia / metabolism
Time Factors
Up-Regulation
fas Receptor / biosynthesis
fms-Like Tyrosine Kinase 3

Substances

CD11 Antigens
CD8 Antigens
CD8alpha antigen
Integrin alphaXbeta2
Membrane Proteins
Proto-Oncogene Proteins
RNA, Messenger
fas Receptor
flt3 ligand protein
Flt3 protein, mouse
Proto-Oncogene Proteins c-kit
Receptor Protein-Tyrosine Kinases
fms-Like Tyrosine Kinase 3