Abstract
STAT6 plays an important role in IL-4-mediated B cell activation and differentiation. To identify primary and secondary target genes of STAT6, gene expression profiles of IL-4-stimulated B cells from STAT6+/+ vs STAT6-/- mice were compared. Statistical analysis revealed that 106 distinct probe sets including 70 known genes were differentially expressed between the 2 genotypes. These genes include transcription factors, kinases, and other enzymes, cell surface receptors, and Ig H chains. Surprisingly, although 31 genes were expressed at higher levels in STAT6+/+ B cells, 39 genes were expressed at higher abundance in STAT6-/- B cells. This result implies both positive and negative regulatory functions of STAT6 in IL-4-mediated gene expression. Furthermore, IL-4 induces expression of the transcription factor Krox20, which is required for maximal IL-4-induced transcription.
MeSH terms
-
Animals
-
B-Lymphocytes / cytology
-
B-Lymphocytes / immunology*
-
B-Lymphocytes / metabolism*
-
Cell Differentiation / genetics
-
Cell Differentiation / immunology
-
Cells, Cultured
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / physiology
-
Down-Regulation / genetics
-
Down-Regulation / immunology*
-
Drug Combinations
-
Early Growth Response Protein 2
-
Gene Expression Regulation / immunology*
-
Interleukin-4 / pharmacology*
-
Lipopolysaccharides / pharmacology
-
Lymphocyte Activation / genetics
-
Mice
-
Mice, Inbred BALB C
-
Mice, Knockout
-
Receptors, IgE / biosynthesis
-
Receptors, IgE / genetics
-
STAT6 Transcription Factor
-
Signal Transduction / genetics
-
Signal Transduction / immunology*
-
Trans-Activators / genetics
-
Trans-Activators / physiology*
-
Transcription Factors / genetics
-
Transcription Factors / physiology
-
Transduction, Genetic
-
Up-Regulation / genetics
-
Up-Regulation / immunology*
Substances
-
DNA-Binding Proteins
-
Drug Combinations
-
Early Growth Response Protein 2
-
Egr2 protein, mouse
-
Lipopolysaccharides
-
Receptors, IgE
-
STAT6 Transcription Factor
-
Stat6 protein, mouse
-
Trans-Activators
-
Transcription Factors
-
Interleukin-4