Abstract
Using a subtraction method, we have isolated genes that are induced early in the differentiation of mouse 3T3-L1 preadipocyte cells into adipocytes. These include the genes encoding transcription factors and signalling proteins, as well as unknown genes. Bach1, a transcription factor, and ARA70, a cofactor, were rapidly induced during differentiation. The induction of these two genes was observed only in growth-arrested 3T3-L1 cells, and not in proliferating cells. In NIH-3T3 cells, no induction was observed under either set of conditions. These results strongly indicate that Bach1 and ARA70 have valuable roles at the onset of adipocyte differentiation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
3T3 Cells
-
Adipocytes / cytology*
-
Animals
-
Basic-Leucine Zipper Transcription Factors
-
Blotting, Northern
-
Cell Differentiation
-
Cell Division
-
Cloning, Molecular
-
DNA, Complementary / metabolism
-
Mice
-
Mice, Inbred C3H
-
Nuclear Receptor Coactivators
-
Nucleic Acid Hybridization
-
Oncogene Proteins*
-
RNA / metabolism
-
RNA, Messenger / metabolism
-
Signal Transduction
-
Time Factors
-
Trans-Activators / biosynthesis*
-
Transcription Factors / biosynthesis*
Substances
-
Bach1 protein, mouse
-
Basic-Leucine Zipper Transcription Factors
-
DNA, Complementary
-
NCOA4 protein, human
-
Nuclear Receptor Coactivators
-
Oncogene Proteins
-
RNA, Messenger
-
Trans-Activators
-
Transcription Factors
-
RNA