Abstract
A 260-residue protein (FabG3) encoded by the Rv2002 gene of Mycobacterium tuberculosis shows amino-acid sequence similarity to beta-ketoacyl carrier protein (ACP) reductase, FabG. A soluble mutant (I6T/V47M/T69M) was produced by the green fluorescent protein-based directed-evolution method. It was crystallized at 296 K using the hanging-drop vapour-diffusion method. The diffraction quality of the crystal improved significantly after annealing/dehydration. X-ray diffraction data were collected to 1.8 A resolution using synchrotron radiation. The crystal belongs to the space group P3(1)21 (or P3(2)21), with unit-cell parameters a = b = 70.38, c = 148.93 A. The asymmetric unit contains two subunits, with a corresponding V(M) of 1.90 A(3) Da(-1) and a solvent content of 35.3%.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3-Oxoacyl-(Acyl-Carrier-Protein) Reductase
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Alcohol Oxidoreductases / chemistry*
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Alcohol Oxidoreductases / genetics*
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Alcohol Oxidoreductases / metabolism
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Bacterial Proteins*
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Cloning, Molecular
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Crystallization
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Crystallography, X-Ray
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Escherichia coli / genetics
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Green Fluorescent Proteins
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Luminescent Proteins / genetics
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Luminescent Proteins / isolation & purification
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Luminescent Proteins / metabolism
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Models, Molecular
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Mutation
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Mycobacterium tuberculosis / chemistry
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Mycobacterium tuberculosis / genetics*
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Protein Conformation
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Protein Engineering / methods
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Sequence Homology, Amino Acid
Substances
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Bacterial Proteins
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Luminescent Proteins
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Green Fluorescent Proteins
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Alcohol Oxidoreductases
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3-Oxoacyl-(Acyl-Carrier-Protein) Reductase
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Rv2002 protein, Mycobacterium tuberculosis