Objective: To detect the expression of amelogenin (Am) gene in Escherichia coli (E. coli).
Methods: The cDNA fragmain of Am gene was obtained with EcoRI and Xhol I from the plasmid PUC18/Am. The fragment was inserted into prokaryotic gene fusion vector PGEX-4T-2 and an expression plasmid PGEX-4T-2/Am was constructed. PGEX-4T-2/Am was induced by IPTG for 4 h.
Results: 15% SDS-PAGE revealed a new forein protein band near 27,000.
Conclusion: The constructed plasmid expresses Am gene in E. coli.