Abstract
The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shows that KCO1 is involved in the formation of SV channels.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / chemistry
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Arabidopsis / cytology
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Arabidopsis / genetics
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Electric Conductivity
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Gene Deletion
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Gene Expression Profiling
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Green Fluorescent Proteins
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Intracellular Membranes / chemistry
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Intracellular Membranes / metabolism
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Luminescent Proteins / metabolism
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Nicotiana
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Organ Specificity
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Patch-Clamp Techniques
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Potassium Channels / chemistry*
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Potassium Channels / genetics
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Potassium Channels / metabolism*
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Potassium Channels, Tandem Pore Domain*
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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RNA, Plant / analysis
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RNA, Plant / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Vacuoles / chemistry*
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Vacuoles / metabolism
Substances
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Arabidopsis Proteins
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KCO1 protein, Arabidopsis
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Luminescent Proteins
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Potassium Channels
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Potassium Channels, Tandem Pore Domain
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RNA, Messenger
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RNA, Plant
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Green Fluorescent Proteins