Abstract
Advanced glycation end products (AGE) are known to serve as ligands for the scavenger receptors such as SR-A, CD36 and SR-BI. In the current study, we examined whether AGE is recognized by lectin-like oxidized low density lipoprotein receptor-1 (LOX-1). Cellular binding experiments revealed that AGE-bovine serum albumin (AGE-BSA) showed the specific binding to CHO cells overexpressing bovine LOX-1 (BLOX-1), which was effectively suppressed by an anti-BLOX-1 antibody. Cultured bovine aortic endothelial cells also showed the specific binding for AGE-BSA, which was suppressed by 67% by the anti-BLOX-1 antibody. Thus, LOX-1 is identified as a novel endothelial receptor for AGE.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aorta / cytology
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Binding, Competitive
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CHO Cells
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Cattle
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Cells, Cultured
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Cricetinae
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Endocytosis
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Endothelium / metabolism*
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Glycation End Products, Advanced / metabolism*
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Receptor for Advanced Glycation End Products
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Receptors, Immunologic / metabolism
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Receptors, LDL / genetics
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Receptors, LDL / immunology
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Receptors, LDL / metabolism*
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Receptors, Oxidized LDL
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Scavenger Receptors, Class E
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Serum Albumin, Bovine / metabolism
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Transfection
Substances
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Glycation End Products, Advanced
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Receptor for Advanced Glycation End Products
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Receptors, Immunologic
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Receptors, LDL
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Receptors, Oxidized LDL
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Scavenger Receptors, Class E
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Serum Albumin, Bovine