Cutting edge: Immune stimulation by neisserial porins is toll-like receptor 2 and MyD88 dependent

J Immunol. 2002 Feb 15;168(4):1533-7. doi: 10.4049/jimmunol.168.4.1533.

Abstract

The immunopotentiating activity of neisserial porins, the major outer membrane protein of the pathogenic Neisseria, is mediated by its ability to stimulate B cells and up-regulate the surface expression of B7-2. This ability is dependent on MyD88 and Toll-like receptor (TLR)2 expression, as demonstrated by a lack of a response by B cells from MyD88 or TLR2 knockout mice to the porins. Using previously described TLR2-dependent reporter constructs, these results were confirmed and were shown to be due to induction of NF-kappaB nuclear translocation. This is the first demonstration of known vaccine adjuvant to stimulate immune cells via TLR2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Adaptor Proteins, Signal Transducing
  • Animals
  • Antigens, Bacterial / immunology
  • Antigens, CD / metabolism
  • Antigens, Differentiation / genetics
  • Antigens, Differentiation / physiology*
  • B-Lymphocytes / immunology*
  • B7-2 Antigen
  • CHO Cells
  • Cell Line
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Cricetinae
  • Drosophila Proteins*
  • Histocompatibility Antigens Class II / metabolism
  • Humans
  • Lymphocyte Activation
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Membrane Glycoproteins / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Myeloid Differentiation Factor 88
  • NF-kappa B / metabolism
  • Neisseria meningitidis / immunology*
  • Porins / immunology*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / physiology*
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / physiology*
  • Toll-Like Receptor 2
  • Toll-Like Receptors

Substances

  • Adaptor Proteins, Signal Transducing
  • Antigens, Bacterial
  • Antigens, CD
  • Antigens, Differentiation
  • B7-2 Antigen
  • CD86 protein, human
  • Cd86 protein, mouse
  • Drosophila Proteins
  • Histocompatibility Antigens Class II
  • MYD88 protein, human
  • Membrane Glycoproteins
  • Myd88 protein, mouse
  • Myeloid Differentiation Factor 88
  • NF-kappa B
  • Porins
  • Receptors, Cell Surface
  • Receptors, Immunologic
  • TLR2 protein, human
  • Toll-Like Receptor 2
  • Toll-Like Receptors