Evolution of IgM, IgE and IgG(1-4 )antibody responses in early childhood monitored with recombinant allergen components: implications for class switch mechanisms

Eur J Immunol. 2002 Feb;32(2):576-84. doi: 10.1002/1521-4141(200202)32:2<576::AID-IMMU576>3.0.CO;2-U.

Abstract

The formation of IgE antibodies against environmental allergens represents the hallmark of type I allergy. Data from in vitro cultured cells and experimental animal models provide controversial evidence for isotype switching from IgM to IgE production via sequential as well as non-sequential (i.e. direct) class switch. We analyzed the evolution of IgE responses in 11 children developing birch pollen and/or grass pollen allergy during the first 7 years of life using purified recombinant allergen molecules (major birch pollen allergen, Bet v 1; major timothy grass pollen allergens, Phl p 1, Phl p 2, Phl p 5). Demographic, clinical and serological data indicated a postnatal sensitization to pollen allergens. A parallel development of IgG(1-4) and IgE responses to recombinant allergen molecules compatible with a strictly sequential class switch to IgE was observed only in one child. The only partly synchronized and dissociated development of allergen-specific antibody responses found in all other cases can be best explained by a partly sequential class switch involving few switch stations or, more likely, by direct class switching. Kinetics and courses of allergen-specific antibody responses (IgM, IgG(1-4), IgE) during the first years of life suggest that, once established, allergen-specific IgE responses are driven by antigen contact rather than by cytokines controlling class switch to IgE.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Allergens / immunology*
  • Animals
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Hypersensitivity, Immediate / etiology*
  • Hypersensitivity, Immediate / genetics
  • Hypersensitivity, Immediate / immunology*
  • Immunoglobulin Class Switching*
  • Immunoglobulin E / biosynthesis
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / classification
  • Immunoglobulin M / biosynthesis
  • Infant
  • Male
  • Models, Immunological
  • Pollen / immunology
  • Rhinitis, Allergic, Seasonal / etiology
  • Rhinitis, Allergic, Seasonal / genetics
  • Rhinitis, Allergic, Seasonal / immunology

Substances

  • Allergens
  • Immunoglobulin G
  • Immunoglobulin M
  • Immunoglobulin E