Abstract
Conditions of achieving the maximal accumulation of sulfhydryl metabolites in the leaves of tobacco were explored. Simultaneous production of bacterial O-acetylserine (thiol)-lyase and serine acetyltransferase resulted in the increased thiols contents as compared to single transformants and controls. However, leaf discs feeding experiments differently affected thiols concentration in different plant groups and suggested that the most promising strategy to obtain plants with a high level of non-protein thiol-containing compounds might be sulfate feeding to plants overproducing serine acetyltransferase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyltransferases / genetics
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Acetyltransferases / metabolism*
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Agrobacterium tumefaciens / genetics
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Blotting, Western
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Carbon-Oxygen Lyases / genetics
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Carbon-Oxygen Lyases / metabolism*
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Cysteine / metabolism*
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Cysteine Synthase
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Electroporation
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Kanamycin / metabolism
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Multienzyme Complexes*
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Nicotiana / genetics*
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Nicotiana / metabolism*
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Plants, Genetically Modified
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Saccharomyces cerevisiae Proteins*
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Serine O-Acetyltransferase
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Sulfhydryl Compounds / metabolism*
Substances
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Bacterial Proteins
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Multienzyme Complexes
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Saccharomyces cerevisiae Proteins
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Sulfhydryl Compounds
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Kanamycin
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Acetyltransferases
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Serine O-Acetyltransferase
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Cysteine Synthase
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MET17 protein, S cerevisiae
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O-acetylhomoserine (thiol)-lyase
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Carbon-Oxygen Lyases
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Cysteine