Increased expression of cardiotrophin-1 during ventricular remodeling in hypertensive rats

Am J Physiol Heart Circ Physiol. 2002 Mar;282(3):H896-901. doi: 10.1152/ajpheart.00591.2001.

Abstract

Cardiotrophin-1 (CT-1) stimulates longitudinal myocardial cell hypertrophy. We examined the expression of CT-1, leukemia inhibitory factor (LIF), and gp130 by competitive RT-PCR and Western blotting in Dahl salt-sensitive (DS) rats with a high-salt diet, which showed a distinct transition from left ventricular hypertrophy (LVH) to congestive heart failure (CHF). The expression levels of CT-1 mRNA and protein were significantly increased at the CHF stage compared with the LVH stage and age-matched Dahl salt-resistant (DR) rats (n = 6 for each group). mRNA expression of LIF was not changed in the left ventricle at any stage by RT-PCR. gp130 mRNA and protein levels of DS rats at 11 and 17 wk were significantly increased compared with age-matched DR rats. The isolated myocyte length of DS rats at 17 wk was the longest among the four groups of rats. The LV end-diastolic dimension (LVDd) of DS rats, determined by echocardiography, was significantly increased at the CHF stage. There was a significant correlation between the CT-1 protein level and LVDd. CT-1 may play a role in ventricular remodeling during transition from LVH to CHF in the rat hypertensive model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Pressure
  • Cell Division
  • Cells, Cultured
  • Contactins
  • Cytokines / genetics*
  • Disease Models, Animal
  • Echocardiography
  • Gene Expression Regulation*
  • Growth Inhibitors / genetics
  • Heart / physiology*
  • Heart Failure / genetics
  • Heart Failure / physiopathology
  • Heart Ventricles
  • Hemodynamics / physiology*
  • Hypertrophy, Left Ventricular / genetics
  • Hypertrophy, Left Ventricular / physiopathology
  • Interleukin-6*
  • Leukemia Inhibitory Factor
  • Lymphokines / genetics
  • Male
  • Myocardial Contraction
  • Myocardium / metabolism*
  • Neural Cell Adhesion Molecules / genetics
  • Rats
  • Rats, Inbred Dahl
  • Regression Analysis
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Contactins
  • Cytokines
  • Growth Inhibitors
  • Interleukin-6
  • Leukemia Inhibitory Factor
  • Lymphokines
  • Neural Cell Adhesion Molecules
  • cardiotrophin 1