Expression of Fas ligand in patients with evident skull base involvement of nasopharyngeal carcinoma

Ming-Hsui Tsai; Kuan-Chih Chow; Tze-Yi Lin; Su-Peng Yeh; Chung-Tsen Hsueh; Kwan-Hwa Chi

Expression of Fas ligand in patients with evident skull base involvement of nasopharyngeal carcinoma

Oncol Rep. 2002 Mar-Apr;9(2):247-51.

Authors

Ming-Hsui Tsai¹, Kuan-Chih Chow, Tze-Yi Lin, Su-Peng Yeh, Chung-Tsen Hsueh, Kwan-Hwa Chi

Affiliation

¹ Department of Otolaryngology, China Medical College Hospital and Institute of Medical Research, Taichung, Taiwan.

PMID: 11836588

Abstract

We investigated whether skull base involvement in patients with nasopharyngeal carcinoma (NPC) is correlated with expression of Fas ligand (FasL) in NPC cells. A prospective assessment of FasL expression was determined by immunohistochemistry and in situ hybridization in 98 patients with newly diagnosed NPC. Among these patients, 21 had evident skull base involvement. Expressions of human apoptosis-related genes and FasL were confirmed by reverse transcription-polymerase chain reaction. Relation between the frequency of skull base involvement and FasL expression was analyzed by Chi-square and multivariate analyses. FasL expression was detected in 32 (32.6%) of 98 pathological sections. Compared to patients with low FasL expression in tumors, patients with notable FasL expression had higher incidence of skull base involvement (28.6 vs. 71.4%, p<0.005). Expression of FasL in tumor cells was correlated with the higher frequency of skull base involvement in patients with NPC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
DNA Primers / chemistry
Fas Ligand Protein
Female
Humans
Immunoenzyme Techniques
In Situ Hybridization
Male
Membrane Glycoproteins / genetics*
Membrane Glycoproteins / metabolism
Middle Aged
Monomeric GTP-Binding Proteins / metabolism
NM23 Nucleoside Diphosphate Kinases
Nasopharyngeal Neoplasms / metabolism*
Nasopharyngeal Neoplasms / pathology
Neoplasm Staging
Nucleoside-Diphosphate Kinase*
Prospective Studies
Proto-Oncogene Proteins c-bcl-2 / metabolism
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Skull Base Neoplasms / metabolism*
Skull Base Neoplasms / pathology
Transcription Factors / metabolism
Tumor Suppressor Protein p53 / metabolism
fas Receptor / genetics
fas Receptor / metabolism

Substances

DNA Primers
FASLG protein, human
Fas Ligand Protein
Membrane Glycoproteins
NM23 Nucleoside Diphosphate Kinases
Proto-Oncogene Proteins c-bcl-2
RNA, Messenger
Transcription Factors
Tumor Suppressor Protein p53
fas Receptor
Nucleoside-Diphosphate Kinase
Monomeric GTP-Binding Proteins