Abstract
We demonstrate that base and sugar protons within a DNA oligomer can be assigned unambiguously following site-specific 15N,13C isotope incorporation at levels as low as 1% enrichment. This simple and cost-effective methodology is demonstrated on the d(GGGTTCAGG) DNA sequence, which forms a dimeric G-quadruplex containing G.G.G.G tetrads sandwiched between G.(C-A) triads.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Carbon Isotopes / chemistry
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DNA / chemistry*
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Isotope Labeling / methods*
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Nitrogen Isotopes / chemistry
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Nuclear Magnetic Resonance, Biomolecular / methods*
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Organophosphorus Compounds / chemistry
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Sequence Analysis, DNA / methods*
Substances
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Carbon Isotopes
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Nitrogen Isotopes
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Organophosphorus Compounds
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phosphoramidite
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DNA