Abstract
The Nedd8 ubiquitin-like protein modification pathway regulates cell-cycle progression. Our analysis of Nedd8 requirements during Caenorhabditis elegans embryogenesis indicates that the cytoskeleton is another target. Nedd8 conjugation negatively regulated contractility of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis. The Nedd8 pathway also was required after meiosis to negatively regulate katanin, a microtubule-severing complex, permitting the assembly of a large mitotic spindle. We propose that Nedd8-modified cullin, as part of an E3 ubiquitin ligase complex, targets katanin for degradation during the transition from meiosis to mitosis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actin Cytoskeleton / physiology*
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Actin Cytoskeleton / ultrastructure
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism*
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Animals
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Caenorhabditis elegans / embryology*
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism
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Cell Division
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Cell Membrane / ultrastructure
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Cell Nucleus / physiology
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Cell Nucleus / ultrastructure
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Cullin Proteins*
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Embryo, Nonmammalian / metabolism
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Genes, Helminth
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Katanin
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Meiosis
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Microtubules / drug effects
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Microtubules / physiology*
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Microtubules / ultrastructure
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Mitosis
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Mutation
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Nocodazole / pharmacology
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RNA, Helminth / genetics
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Spindle Apparatus / physiology
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Spindle Apparatus / ultrastructure
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Ubiquitins / genetics
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Ubiquitins / metabolism*
Substances
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CUL2 protein, human
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CUL3 protein, human
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Caenorhabditis elegans Proteins
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Cell Cycle Proteins
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Cullin Proteins
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RNA, Helminth
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Ubiquitins
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Adenosine Triphosphatases
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MEI-1 protein, C elegans
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Katanin
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Nocodazole