[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3']

T A Ushakova; L I Puchkova; V V Gutorov; V N Kuvshinov; V I Repin

[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3']

Prikl Biokhim Mikrobiol. 2002 Jan-Feb;38(1):25-8.

[Article in Russian]

Authors

T A Ushakova¹, L I Puchkova, V V Gutorov, V N Kuvshinov, V I Repin

Affiliation

¹ State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Oblast, 630559 Russia.

PMID: 11852562

Abstract

A new restriction endonuclease Sst12I belonging to the II type and recognizing the sequence 5'-CTGCAG-3' was isolated from the bacterial strain Streptomyces sp. St-12. The enzyme hydrolyzes DNA between adenine and guanine residues; thus, it is a true isoschizomer of restrictase PstI. In contrast to PstI, the restriction endonuclease Sst12I hydrolyses DNA both at 37 degrees and 55 degrees C and remains active after long-term storage.

Publication types

Comparative Study
English Abstract

MeSH terms

Bacteriophage lambda / genetics
DNA Restriction Enzymes / classification
DNA Restriction Enzymes / isolation & purification*
DNA, Viral / metabolism
Deoxyribonucleases, Type II Site-Specific / metabolism*
Enzyme Stability
Hydrolysis
Streptomyces / enzymology*
Substrate Specificity
Temperature

Substances

DNA, Viral
DNA Restriction Enzymes
CTGCAG-specific type II deoxyribonucleases
Deoxyribonucleases, Type II Site-Specific