Array-based electrical detection of DNA with nanoparticle probes

Science. 2002 Feb 22;295(5559):1503-6. doi: 10.1126/science.1067003.

Abstract

A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration-dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of approximately 100,000:1.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antigens, Bacterial*
  • Bacillus anthracis / genetics
  • Bacillus anthracis / isolation & purification*
  • Bacterial Toxins / genetics
  • Bacteriological Techniques
  • DNA, Bacterial / analysis*
  • Electric Conductivity*
  • Electronics
  • Gold
  • Microelectrodes
  • Nanotechnology*
  • Nucleic Acid Hybridization*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Oligonucleotide Probes
  • Point Mutation
  • Sensitivity and Specificity
  • Silver

Substances

  • Antigens, Bacterial
  • Bacterial Toxins
  • DNA, Bacterial
  • Oligonucleotide Probes
  • anthrax toxin
  • Silver
  • Gold