Abstract
Two genes, originally identified in genetic screens for Caenorhabditis elegans mutants that arrest in metaphase of meiosis I, prove to encode subunits of the anaphase-promoting complex or cyclosome (APC/C). RNA interference studies reveal that these and other APC/C subunits are essential for the segregation of chromosomal homologs during meiosis I. Further, chromosome segregation during meiosis I requires APC/C functions in addition to the release of sister chromatid cohesion.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Anaphase-Promoting Complex-Cyclosome
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Animals
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Caenorhabditis elegans / embryology
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / physiology
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Chromosome Segregation / physiology
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DNA Mutational Analysis
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Fungal Proteins / genetics
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Fungal Proteins / physiology
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Ligases / genetics*
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Ligases / physiology
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Meiosis / physiology*
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Molecular Sequence Data
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Mutation / genetics
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Phosphoproteins*
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Protein Subunits
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RNA, Double-Stranded / genetics
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RNA, Double-Stranded / physiology
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Schizosaccharomyces pombe Proteins*
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Sequence Alignment
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Suppression, Genetic / genetics
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Suppression, Genetic / physiology
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Ubiquitin-Protein Ligase Complexes*
Substances
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Fungal Proteins
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Phosphoproteins
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Protein Subunits
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RNA, Double-Stranded
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Schizosaccharomyces pombe Proteins
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REC8 protein, S pombe
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Ubiquitin-Protein Ligase Complexes
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Anaphase-Promoting Complex-Cyclosome
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Ligases