The yeast clathrin adaptor protein complex 1 is required for the efficient retention of a subset of late Golgi membrane proteins

Dev Cell. 2002 Mar;2(3):283-94. doi: 10.1016/s1534-5807(02)00127-2.

Abstract

In yeast, certain resident trans-Golgi network (TGN) proteins achieve steady-state localization by cycling through late endosomes. Here, we show that chitin synthase III (Chs3p), an enzyme involved in the assembly of the cell wall at the mother-bud junction, populates an intracellular reservoir that is maintained by a cycle of transport between the TGN and early endosomes. Traffic of Chs3p from the TGN/early endosome to the cell surface requires CHS5 and CHS6, mutant alleles of which trap Chs3p in the TGN/early endosome. Disruption of the clathrin adaptor protein complex 1 (AP-1) restores Chs3p transport to the plasma membrane. Similarly, in AP-1 deficient cells, the resident TGN/early endosome syntaxin, Tlg1p, is missorted. We propose that clathrin and AP-1 act to recycle Chs3p and Tlg1p from the early endosome to the TGN.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Vesicular Transport
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Chitin Synthase / metabolism*
  • Endosomes / metabolism
  • Fungal Proteins / metabolism*
  • Gene Expression Regulation, Fungal
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism*
  • Mutation / physiology
  • Protein Transport / physiology
  • Qa-SNARE Proteins
  • Saccharomyces cerevisiae
  • Secretory Vesicles / metabolism
  • trans-Golgi Network / metabolism*

Substances

  • Adaptor Proteins, Vesicular Transport
  • Carrier Proteins
  • Fungal Proteins
  • Membrane Proteins
  • Qa-SNARE Proteins
  • Chitin Synthase