Insulin-induced expression of the activity-regulated cytoskeleton-associated gene (ARC) in human neuroblastoma cells requires p21(ras), mitogen-activated protein kinase/extracellular regulated kinase and src tyrosine kinases but is protein kinase C-independent

Neurosci Lett. 2002 Mar 22;321(3):153-6. doi: 10.1016/s0304-3940(01)02532-0.

Abstract

We examined the effect of insulin on the expression of the activity-regulated cytoskeleton-associated gene (ARC), an effector immediate early gene with a proposed role in memory formation. In human SH-SY5Y neuroblastoma cells, application of insulin leads to dramatic increase in ARC mRNA and protein levels. Inhibition experiments reveal, that p21(ras), mitogen-activated protein kinase/extracellular regulated kinase and tyrosine kinase (src) activity are required for the insulin-induced ARC expression in SH-SY5Y cells, whereas protein kinase C is not involved in the signal transduction pathway. Our data indicated for the first time a correlation of the insulin-controlled signal cascade and the induction of synaptic plasticity-associated immediate early genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Central Nervous System / enzymology
  • Central Nervous System / growth & development
  • Cytoskeletal Proteins / drug effects
  • Cytoskeletal Proteins / genetics*
  • Cytoskeletal Proteins / metabolism
  • Enzyme Inhibitors / pharmacology
  • Gene Expression Regulation, Developmental / physiology
  • Humans
  • Insulin / metabolism*
  • Insulin / pharmacology
  • Memory / physiology
  • Mitogen-Activated Protein Kinases / metabolism*
  • Nerve Tissue Proteins / drug effects
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Neuroblastoma
  • Neuronal Plasticity / genetics
  • Neurons / drug effects
  • Neurons / enzymology*
  • Protein Kinase C / metabolism*
  • Proto-Oncogene Proteins p21(ras) / metabolism*
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism
  • Signal Transduction / genetics
  • Synapses / metabolism
  • Transcription, Genetic / drug effects
  • Transcription, Genetic / physiology
  • Tumor Cells, Cultured
  • src-Family Kinases / metabolism*

Substances

  • Cytoskeletal Proteins
  • Enzyme Inhibitors
  • Insulin
  • Nerve Tissue Proteins
  • RNA, Messenger
  • activity regulated cytoskeletal-associated protein
  • src-Family Kinases
  • Protein Kinase C
  • Mitogen-Activated Protein Kinases
  • HRAS protein, human
  • Proto-Oncogene Proteins p21(ras)