Soluble complement receptor type 1 protects rats from lethal shock induced by anti-Crry antibody following lipopolysaccharide priming

Int Arch Allergy Immunol. 2002 Jan;127(1):55-62. doi: 10.1159/000048169.

Abstract

Background: In rats primed with a trace amount of lipopolysaccharide (LPS), acute lethal shock is induced following the injection of monoclonal antibody against a membrane inhibitor of complement (anti-Crry). Administration of cobra venom factor to exhaust complement before the LPS priming can prevent the lethal reaction. Therefore, we evaluated whether soluble complement receptor type 1 (sCR1), which inhibits complement reaction, can interfere with lethal shock when administered after LPS priming or even after anti-Crry injection.

Methods: sCR1 was administered intravenously before or after the administration of anti-Crry, and the effects on blood pressure and acute lethality were determined.

Results: Administration of sCR1 could rescue rats from lethal shock even when it was administered after anti-Crry injection, which immediately causes a blood pressure decrease leading to lethal shock.

Conclusion: sCR1 may be an effective treatment for acute shock involving complement activation.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / administration & dosage*
  • Antibodies, Monoclonal / immunology
  • Antigens, Surface
  • Complement Activation
  • Fluorescent Antibody Technique
  • Humans
  • Lipopolysaccharides / administration & dosage*
  • Male
  • Rats
  • Rats, Wistar
  • Receptors, Cell Surface
  • Receptors, Complement / immunology*
  • Receptors, Complement / metabolism
  • Receptors, Complement 3b / administration & dosage*
  • Recombinant Proteins / administration & dosage
  • Shock, Septic / etiology
  • Shock, Septic / prevention & control*
  • Solubility

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Cr1l protein, rat
  • Lipopolysaccharides
  • Receptors, Cell Surface
  • Receptors, Complement
  • Receptors, Complement 3b
  • Recombinant Proteins