Objective. A software used for acquiring and analyzing signals was developed for ergonomical research on Human Workload in space manual system. Method. As an important part of the whole experimental equipment and being developed in PC, the software is composed of acquisition and display program, analysis and processing program and data files. Result. The software is capable of making realtime acquisition and display of four channels of myoelectric potential signals and one channel of operation signal synchronously. The signals are then analyzed and processed off-line. Conclusion. During the development, its realtime feature was implemented by means of some technical methods, such as that different machine assigned to view-scenery display and signal acquisition, different frequency assigned to EMG signal and operation ones, and different cycle assigned to acquisition, display and storage. Above all, with friendly man-machine interface and high accuracy of data, the software was even reliable.