Alternative transcripts of the candidate tumor suppressor gene, WWOX, are expressed at high levels in human breast tumors

Oncogene. 2002 Mar 14;21(12):1832-40. doi: 10.1038/sj.onc.1205273.

Abstract

The presence of putative tumor-suppressor genes on chromosome 16q23.2-24.1 has been suggested by LOH analysis in several cancer types. This region overlaps with the fragile site FRA16D and the region of homozygous deletions found in several cancer types. The candidate gene WWOX/FOR has been mapped within this region. The mouse homologue of the WWOX protein has been defined as an apoptogenic protein and an essential partner of p53 in cell death, supporting WWOX as a tumor suppressor gene candidate. We performed an expression study of the WWOX/FOR gene in a series of human breast tumors and breast cancer cell lines, and detected reduced expression of the WWOX/FOR transcript in a series of breast cancer cells. Furthermore, identification of two distinct alternative WWOX transcripts expressed at high levels in human tumors suggests an involvement of the WWOX gene in breast cancer progression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Alternative Splicing / genetics
  • Base Sequence
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Chromosomes, Human, Pair 16 / genetics
  • CpG Islands
  • DNA Primers / chemistry
  • Female
  • Gene Deletion
  • Gene Expression Regulation, Neoplastic / genetics*
  • Genes, Tumor Suppressor*
  • Humans
  • Loss of Heterozygosity
  • Microsatellite Repeats
  • Middle Aged
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / metabolism
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transfection
  • Tumor Cells, Cultured
  • Up-Regulation

Substances

  • Carrier Proteins
  • DNA Primers
  • Neoplasm Proteins
  • RNA, Messenger

Associated data

  • GENBANK/AW874683
  • GENBANK/AW874684
  • GENBANK/AW874685
  • GENBANK/AW874686
  • GENBANK/AW874687
  • GENBANK/AW874688
  • GENBANK/AW874689
  • GENBANK/AW874690
  • GENBANK/AW874691
  • GENBANK/AW874692
  • GENBANK/AW874693
  • GENBANK/AW874694
  • GENBANK/AW874695
  • GENBANK/AW874696
  • GENBANK/AW874697
  • GENBANK/AW874698
  • GENBANK/AW874699
  • GENBANK/AW874700
  • GENBANK/AZ124367
  • GENBANK/AZ124368
  • GENBANK/AZ124369
  • GENBANK/AZ124370
  • GENBANK/AZ124371
  • GENBANK/AZ124372
  • GENBANK/AZ124373
  • GENBANK/AZ124374
  • GENBANK/AZ124375
  • GENBANK/AZ124376
  • GENBANK/AZ124377
  • GENBANK/AZ124378
  • GENBANK/AZ124379
  • GENBANK/AZ124382