Abstract
The search for antiviral agents against vesicular stomatitis virus, herpes simplex virus type 1 and poliovirus type 2 in plants extracts, led to the isolation of two antipoliovirus flavonoids from the medicinal plant Psiadia dentata (Cass.) DC, Asteraceae: 3-methylkaempferol and 3,4'-dimethylkaempferol. The antipoliovirus activity of both compounds was estimated by comparison with 3-methylquercetin, guanidine and Ro-090179. The most potent inhibitor of poliovirus replication was 3-methylkaempferol, and therefore we investigated its mechanism of action. We showed, using the inhibition of [3H]uridine incorporation in viral RNA and performing a dot-blot with one RNA probe specific for the poliovirus genomic strand RNA, that 3-methylkaempferol inhibits the genomic RNA synthesis of poliovirus.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antiviral Agents / chemistry
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Antiviral Agents / isolation & purification*
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Antiviral Agents / pharmacology*
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Antiviral Agents / toxicity
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Asteraceae / chemistry*
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Chlorocebus aethiops
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Cytopathogenic Effect, Viral / drug effects
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Dose-Response Relationship, Drug
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Flavonoids / chemistry
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Flavonoids / isolation & purification*
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Flavonoids / pharmacology*
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Flavonoids / toxicity
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Flavonols*
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Guanidine / chemistry
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Guanidine / pharmacology
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Kaempferols*
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Molecular Structure
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Plants, Medicinal / chemistry*
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Poliovirus / drug effects*
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Poliovirus / physiology
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Quercetin / analogs & derivatives*
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Quercetin / chemistry
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Quercetin / pharmacology
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RNA, Viral / biosynthesis
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Time Factors
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Vero Cells
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Virus Replication / drug effects
Substances
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3,4'-dimethylkaempferol
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3-methylkaempferol
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Antiviral Agents
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Flavonoids
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Flavonols
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Kaempferols
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RNA, Viral
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3-methylquercetin
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pachypodol
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Quercetin
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Guanidine