Effects of aging on growth factors gene and protein expression in the dorsal and ventral lobes of rat prostate

Biochem Biophys Res Commun. 2002 Mar 29;292(2):482-91. doi: 10.1006/bbrc.2002.6660.

Abstract

We hypothesize that various growth factors and their receptors gene and protein are modulated in dorsal and ventral lobes of aging prostate. To test this hypothesis, TGFbeta1, TGFbeta2 TGFbeta3, TGFbetaR-I, TGFbetaR-II, TGFalpha, EGF, EGFR, KGF and KGFR gene and protein expression were analyzed in dorsal and ventral lobes of aging rat prostates (1, 3, 6, 9, 12, 18, 24, and 28/30 months). KGF gene expression was very weak or absent in 1, 3, and 6 month old rat dorsal and ventral lobes of prostate whereas it re-expressed in 9, 12, 18, 24 and 30 month old rat prostate. All growth factors and their receptors expect KGF and EGFR were mainly localized in epithelium of ventral and dorsal lobes of aging rat prostates. EGF, TGFalpha, TGFbeta1, and TGFbetaR-I protein expression was lacking in stroma of dorsal and ventral lobes of 1, 3, 6, 9, 12/18 months old rat prostates. However, EGF, TGFbeta1 and TGFbetaR-I proteins re-expressed in stroma of 24 and 28 months old rat prostates. KGF protein expression was lacking in epithelium of dorsal and ventral lobes of all aging rat prostates. This is the first report to demonstrate differential gene and protein expression of growth factors in dorsal and ventral lobes is associated with aging rat prostate, suggesting their role in pathogenesis of prostatic diseases with aging.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging*
  • Animals
  • Epidermal Growth Factor
  • ErbB Receptors / biosynthesis
  • ErbB Receptors / genetics
  • ErbB Receptors / immunology
  • Fibroblast Growth Factor 7
  • Fibroblast Growth Factors / biosynthesis
  • Fibroblast Growth Factors / genetics
  • Fibroblast Growth Factors / immunology
  • Growth Substances / biosynthesis*
  • Growth Substances / genetics
  • Immunohistochemistry
  • Kinetics
  • Male
  • Prostate / anatomy & histology
  • Prostate / metabolism*
  • Prostate / physiology
  • RNA, Messenger / biosynthesis
  • Rats
  • Rats, Sprague-Dawley
  • Receptor, Fibroblast Growth Factor, Type 2
  • Receptors, Fibroblast Growth Factor / biosynthesis
  • Receptors, Fibroblast Growth Factor / genetics
  • Receptors, Fibroblast Growth Factor / immunology
  • Receptors, Transforming Growth Factor beta / biosynthesis
  • Receptors, Transforming Growth Factor beta / genetics
  • Receptors, Transforming Growth Factor beta / immunology
  • Transcription, Genetic
  • Transforming Growth Factor alpha / biosynthesis
  • Transforming Growth Factor alpha / genetics
  • Transforming Growth Factor alpha / immunology
  • Transforming Growth Factor beta / biosynthesis
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / immunology

Substances

  • Fgf7 protein, rat
  • Growth Substances
  • RNA, Messenger
  • Receptors, Fibroblast Growth Factor
  • Receptors, Transforming Growth Factor beta
  • Transforming Growth Factor alpha
  • Transforming Growth Factor beta
  • Fibroblast Growth Factor 7
  • Fibroblast Growth Factors
  • Epidermal Growth Factor
  • ErbB Receptors
  • Receptor, Fibroblast Growth Factor, Type 2
  • keratinocyte growth factor receptor