Abstract
A fusion protein of kinesin and gelsolin binds a short actin filament which can be visualized using a standard fluorescence microscope. This technique has provided new insight into the mechanism of kinesin action, and in principle it can be extended to allow single-molecule assays of any protein.
MeSH terms
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Gelsolin / metabolism*
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Green Fluorescent Proteins
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Kinesins / genetics
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Kinesins / metabolism*
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Luminescent Proteins / analysis
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Microscopy, Fluorescence / methods*
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Molecular Biology / methods*
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Phalloidine / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Rhodamines / chemistry
Substances
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Gelsolin
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Luminescent Proteins
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Recombinant Fusion Proteins
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Rhodamines
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Green Fluorescent Proteins
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Phalloidine
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Kinesins