The maximum viable cell density almost kept constant in the experimental range of initial glucose concentrations from 8.9 to 49.6 mmol/L in the batch cultures of recombinant CHO cells. The yield coefficient of lactate to glucose increased with the initial glucose concentrations from 8.9 up to 17.9 mmol/L, and then kept constant with above 17.9 mmol/L. There was little effect of glucose on glutamine metabolism. It was also demonstrated that the accumulative concentration of EPO increased with the increase of glucose concentration from 8.9 to 17.9 mmol/L, and decreased with its further increase from 17.9 to 49.6 mmol/L. It is showed that there is an optimum glucose concentration at which the EPO express of cultured recombinant CHO cells is on the peak.