Expression of progranulin and the epithelin/granulin precursor acrogranin correlates with neoplastic state in renal epithelium

Anticancer Res. 2001 Nov-Dec;21(6A):3739-42.

Abstract

Background: Current traditional pathological parameters, including staging and grading, are not sufficient in predicting outcome in patients with renal cell carcinoma (RCC). Acrogranin is an epithelial growth factor and has been demonstrated to play a role in teratocarcinogenesis and tumorigenesis. The aim of this study was to examine levels of acrogranin in renal cancer.

Materials and methods: Western blot analysis was performed on renal tissue protein lysates. In addition, immunohistochemical (IHC) analysis of acrogranin expression was conducted on tissue sections of various histological types and grades of RCC.

Results: Western analysis showed that acrogranin levels were low in benign renal tissue and increased in malignant renal tissue. In addition, IHC revealed that high-grade RCC exhibited higher levels of expression than low-grade RCC and normal tissue.

Conclusion: These data suggest that acrogranin may be a functional important growth factor in RCC and may be a potential molecular marker for high-grade RCC.

MeSH terms

  • Blotting, Western
  • Carcinoma, Renal Cell / metabolism*
  • Carcinoma, Renal Cell / pathology
  • Glycoproteins / biosynthesis*
  • Growth Substances / biosynthesis*
  • Humans
  • Immunohistochemistry
  • Intercellular Signaling Peptides and Proteins*
  • Kidney / metabolism
  • Kidney Neoplasms / metabolism*
  • Kidney Neoplasms / pathology
  • Progranulins

Substances

  • GRN protein, human
  • Glycoproteins
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Progranulins