Inactivation of the regulatory gene algU or gacA can affect the ability of biocontrol Pseudomonas fluorescens CHA0 to persist as culturable cells in nonsterile soil

Fabio Mascher; Yvan Moënne-Loccoz; Ursula Schnider-Keel; Christoph Keel; Dieter Haas; Geneviève Défago

doi:10.1128/AEM.68.4.2085-2088.2002

Inactivation of the regulatory gene algU or gacA can affect the ability of biocontrol Pseudomonas fluorescens CHA0 to persist as culturable cells in nonsterile soil

Appl Environ Microbiol. 2002 Apr;68(4):2085-8. doi: 10.1128/AEM.68.4.2085-2088.2002.

Authors

Fabio Mascher¹, Yvan Moënne-Loccoz, Ursula Schnider-Keel, Christoph Keel, Dieter Haas, Geneviève Défago

Affiliation

¹ Phytopathology Group, Institute of Plant Science, Swiss Federal Institute of Technology (ETH), CH-8092 Zürich, Switzerland.

Abstract

Rifampin-resistant Pseudomonas fluorescens CHA0-Rif and mutants in which the regulatory gene algU (encoding sigma factor sigma(E)) or gacA (encoding a global regulator of secondary metabolism) was inactivated were compared for persistence in three nonsterile soils. Functional algU and (particularly) gacA were needed for CHA0-Rif to maintain cell culturability in soil.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Colony Count, Microbial
Culture Media
Genes, Regulator / genetics*
Mutation*
Pest Control, Biological
Pseudomonas fluorescens / genetics
Pseudomonas fluorescens / growth & development*
Sigma Factor / genetics*
Soil Microbiology*

Substances

AlgU protein, Pseudomonas aeruginosa
Bacterial Proteins
Culture Media
GacA protein, Bacteria
Sigma Factor