Abstract
A transgenic reporter mouse strain, which expressed the human CD25 (hCD25) surface marker as a reporter under the control of the pre-T cell receptor alpha(pTalpha) promoter, was used to identify lymphoid precursors that expressed pTalpha intracellularly. The hCD25 reporter marked intra- and extrathymic precursors of lymphocytes but not myeloid cells. The earliest intrathymic precursors were CD4(lo)CD8(-)CD25(-)CD44(+)c-Kit(+) cells that expressed elevated levels of Notch-1 mRNA. Clonogenic assays showed that the extrathymic precursors were common lymphoid progenitors (CLPs) that included CD19(-), B220(+), Thy1(+) and CD4(+) cells. Thus, the pTalpha reporter can be used to trace lymphopoiesis between CLPs and alphabeta T cells. The slower extinction of the hCD25 reporter compared to pTalpha enabled us to define points at which pTalpha(-) lineages branched off.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Flow Cytometry
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Gene Expression Regulation
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Hematopoiesis / immunology*
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Humans
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Lymphocytes / cytology
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Lymphocytes / immunology*
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology*
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Membrane Proteins / analysis
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Membrane Proteins / genetics
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Membrane Proteins / immunology
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Phenotype
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Receptor, Notch1
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Receptors, Antigen, T-Cell, alpha-beta
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Receptors, Cell Surface*
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Receptors, Interleukin-2 / immunology
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Reverse Transcriptase Polymerase Chain Reaction
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Stem Cells
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Transcription Factors*
Substances
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Membrane Glycoproteins
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Membrane Proteins
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NOTCH1 protein, human
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Notch1 protein, mouse
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RNA, Messenger
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Receptor, Notch1
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Receptors, Antigen, T-Cell, alpha-beta
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Receptors, Cell Surface
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Receptors, Interleukin-2
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Transcription Factors
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pre-T cell receptor alpha