Cyclophilin a plays distinct roles in human immunodeficiency virus type 1 entry and postentry events, as revealed by spinoculation

J Virol. 2002 May;76(9):4671-7. doi: 10.1128/jvi.76.9.4671-4677.2002.

Abstract

Cyclophilin A (CypA) is necessary for effective human immunodeficiency virus type 1 (HIV-1) replication. However, the functions of CypA and the precise steps at which CypA acts in the HIV-1 life cycle remain to be determined. By using a methodology that bypasses the need for attachment factors-spinoculation-we present evidence that CypA participates in both entry and postentry events.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Centrifugation / methods
  • Cyclophilin A / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • HIV-1 / pathogenicity*
  • HIV-1 / physiology*
  • HeLa Cells
  • Humans
  • Polysaccharide-Lyases / metabolism

Substances

  • Polysaccharide-Lyases
  • heparitinsulfate lyase
  • Cyclophilin A