Enzymatic synthesis of labeled DNA by PCR using new fluorescent thymidine nucleotide analogue and superthermophilic KOD dash DNA polymerase

Tsutomu Obayashi; Mohammed M Masud; Akiko N Ozaki; Hiroaki Ozaki; Masayasu Kuwahara; Hiroaki Sawai

doi:10.1016/s0960-894x(02)00111-7

Enzymatic synthesis of labeled DNA by PCR using new fluorescent thymidine nucleotide analogue and superthermophilic KOD dash DNA polymerase

Bioorg Med Chem Lett. 2002 Apr 22;12(8):1167-70. doi: 10.1016/s0960-894x(02)00111-7.

Authors

Tsutomu Obayashi¹, Mohammed M Masud, Akiko N Ozaki, Hiroaki Ozaki, Masayasu Kuwahara, Hiroaki Sawai

Affiliation

¹ Department of Applied Chemistry, Gunma University, Kiryu, 376-8515, Gunma, Japan.

PMID: 11934580
DOI: 10.1016/s0960-894x(02)00111-7

Abstract

Triphosphate of a new fluorescent labeled thymidine analogue was incorporated as a substrate for PCR using KOD Dash DNA polymerase forming the corresponding fluorescent labeled DNA which is useful for a DNA probe.

MeSH terms

Base Sequence
DNA / chemical synthesis*
DNA Probes
DNA-Directed DNA Polymerase / metabolism*
Fluorescent Dyes / metabolism*
Nucleotides / metabolism*
Polymerase Chain Reaction
Thymidine / metabolism*

Substances

DNA Probes
Fluorescent Dyes
Nucleotides
DNA
DNA-Directed DNA Polymerase
Thymidine