Abstract
Listeria epitope-specific helper T (Th) cells were able to be primed and induced in vivo by immunization with a plasmid carrying an invariant chain (Ii) gene whose class II-associated invariant chain peptide (CLIP) region was replaced by a Listeria Th epitope. Immunization of C3H/He mice with an Ii-LLO 215-226 plasmid induced specific interferon-gamma- and interleukin 2-producing Th cells and conferred significant protective immunity against listerial infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Differentiation, B-Lymphocyte / genetics*
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Antigens, Differentiation, B-Lymphocyte / immunology
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Bacterial Toxins*
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Bacterial Vaccines / immunology*
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Epitopes, T-Lymphocyte*
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Heat-Shock Proteins / genetics*
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Heat-Shock Proteins / immunology
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Hemolysin Proteins
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Histocompatibility Antigens Class II / genetics*
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Histocompatibility Antigens Class II / immunology
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Immunization
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Interferon-gamma / biosynthesis
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Interleukin-2 / biosynthesis
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Listeria monocytogenes / immunology*
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Mice
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Mice, Inbred C3H
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Peptide Fragments / immunology
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Plasmids
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T-Lymphocytes, Helper-Inducer / immunology*
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Vaccines, DNA / immunology*
Substances
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Antigens, Differentiation, B-Lymphocyte
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Bacterial Toxins
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Bacterial Vaccines
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Epitopes, T-Lymphocyte
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Heat-Shock Proteins
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Hemolysin Proteins
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Histocompatibility Antigens Class II
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Interleukin-2
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Peptide Fragments
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Vaccines, DNA
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invariant chain
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Interferon-gamma
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hlyA protein, Listeria monocytogenes