Molecular differentiation between NS1 gene of a field strain Bluetongue virus serotype 2 (BTV-2) and NS1 gene of an attenuated BTV-2 vaccine

Vet Microbiol. 2002 May 24;86(4):337-41. doi: 10.1016/s0378-1135(02)00011-1.

Abstract

At the end of September 2000, clinical symptoms of Bluetongue appeared in sheep flocks of the Balearic Islands (Spain). The presence of the BTV serotype 2 in tissue and blood samples of affected animals was confirmed by laboratory techniques. A systematic vaccination were carried out in affected areas using a live monovalent serotype 2 vaccine available from Onderstepoort laboratory (South Africa). In order to perform epidemiological studies, a new method to differentiate between the NS1 genes of BTV-2 affecting the Balearic islands and that of the Onderstepoort commercial live virus vaccine (monovalent, serotype 2) has been developed. This procedure is based on the use of an RT-PCR, followed by restriction endonuclease analysis. Epidemiological data of a study carried out in the period January-October 2001 using this procedure are included.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bluetongue / diagnosis*
  • Bluetongue / epidemiology
  • Bluetongue / prevention & control
  • Bluetongue virus / classification
  • Bluetongue virus / genetics*
  • Bluetongue virus / immunology
  • DNA Restriction Enzymes
  • Electrophoresis, Agar Gel
  • Genetic Markers
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Sensitivity and Specificity
  • Serotyping
  • Sheep
  • South Africa
  • Spain / epidemiology
  • Vaccination / veterinary
  • Vaccines, Attenuated / genetics
  • Viral Nonstructural Proteins / genetics*
  • Viral Vaccines / genetics*

Substances

  • Genetic Markers
  • NS1 protein, bluetongue virus
  • Vaccines, Attenuated
  • Viral Nonstructural Proteins
  • Viral Vaccines
  • DNA Restriction Enzymes