Abstract
The disaccharide trehalose has been shown to inhibit both bone loss in ovariectomized mice and excessive osteoclastogenesis in lipopolysaccharide-injected mice. However, the mechanism of osteoclastogenesis inhibition by oral administration of trehalose is still unclear. We report here for the first time that a human intestinal epithelial cell line, FHs74Int, also produces osteoprotegerin (OPG) and that trehalose augments OPG production by this cell line. Thus, these results suggest that trehalose promotes the production of OPG by intestinal epithelial cells, which then acts on bone marrow cells, resulting in the suppression of osteoclastogenesis.
MeSH terms
-
Base Sequence
-
DNA Primers
-
Epithelial Cells / drug effects
-
Epithelial Cells / metabolism
-
Glycoproteins / biosynthesis*
-
Glycoproteins / genetics
-
Humans
-
Intestinal Mucosa / metabolism
-
Intestines / cytology
-
Intestines / drug effects*
-
Osteoprotegerin
-
RNA, Messenger / genetics
-
Receptors, Cytoplasmic and Nuclear / biosynthesis*
-
Receptors, Cytoplasmic and Nuclear / genetics
-
Receptors, Tumor Necrosis Factor
-
Reverse Transcriptase Polymerase Chain Reaction
-
Trehalose / pharmacology*
-
Tumor Cells, Cultured
Substances
-
DNA Primers
-
Glycoproteins
-
Osteoprotegerin
-
RNA, Messenger
-
Receptors, Cytoplasmic and Nuclear
-
Receptors, Tumor Necrosis Factor
-
TNFRSF11B protein, human
-
Trehalose