Abstract
According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cloning, Molecular
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Escherichia coli / genetics
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Gene Expression / drug effects
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Isopropyl Thiogalactoside / pharmacology
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Scorpion Venoms / biosynthesis*
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Scorpion Venoms / chemistry
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Scorpion Venoms / genetics*
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Scorpions / chemistry
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Scorpions / genetics*
Substances
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BmK IT(3) scorpion toxin
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Recombinant Proteins
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Scorpion Venoms
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Isopropyl Thiogalactoside