The assignment of PRKCI to bovine chromosome 1q34-->q36 by FISH suggests a new assignment to human chromosome 3

Cytogenet Cell Genet. 2001;95(1-2):79-81. doi: 10.1159/000057021.

Abstract

Two bovine BAC clones were identified by PCR as containing the bovine gene PRKCI. Both clones were assigned by FISH to bands q34-->q36 on BTA1. The sequence information derived from genomic DNA and from both clones was identical and showed a high degree of homology to human PRKCI (HSAXq21.3, 95.5% homology), and mouse Prkcl (MMU3, 13.8 cM, 87.6% homology) and rat Prkcl (88.8% homology). This assignment could suggest a disruption of the synteny conservation of mammalian X-linked genes, but most likely suggests a misassignment of this gene to the human X.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cattle
  • Chromosomes, Human, Pair 3 / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence*
  • Isoenzymes / genetics*
  • Mice
  • Physical Chromosome Mapping*
  • Protein Kinase C / genetics*
  • Rats
  • Reproducibility of Results
  • Sequence Homology, Nucleic Acid
  • Synteny / genetics*
  • X Chromosome / genetics*

Substances

  • Isoenzymes
  • Protein Kinase C
  • protein kinase C lambda