Supercritical fluid extraction and high-performance liquid chromatographic determination of phloroglucinols in St. John's Wort (Hypericum perforatum L.)

Yanyan Cui; Catharina Y W Ang

doi:10.1021/jf011304n

Supercritical fluid extraction and high-performance liquid chromatographic determination of phloroglucinols in St. John's Wort (Hypericum perforatum L.)

J Agric Food Chem. 2002 May 8;50(10):2755-9. doi: 10.1021/jf011304n.

Authors

Yanyan Cui¹, Catharina Y W Ang

Affiliation

¹ Division of Chemistry, National Center for Toxicological Research, U.S. Food and Drug Administration, HFT-230, 3900 NCTR Road, Jefferson, Arkansas 72079, USA.

PMID: 11982394
DOI: 10.1021/jf011304n

Abstract

A small-scale supercritical fluid extraction (SFE) method was developed for the selective extraction of phloroglucinols from St. John's wort (SJW) leaf/flower mixtures using supercritical carbon dioxide (CO(2)). The extraction efficiency was investigated as influenced by pressure, temperature, time, and modifier. The optimized condition of SFE was carried out at 3.80 x 10(4) kpa (5500 psi) and 50 degrees C. Samples were held in static extraction for 10 min, followed by a dynamic extraction for 90 min at the flow rate of 1 mL/min. A simple and sensitive HPLC method was developed for the analysis of hyperforin and adhyperforin, the major phloroglucinols, in the SFE extract of SJW.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Chromatography, High Pressure Liquid*
Chromatography, Supercritical Fluid*
Hypericum / chemistry*
Phloroglucinol / analysis*
Sensitivity and Specificity

Substances

Phloroglucinol