Engineered Listeria monocytogenes as an AIDS vaccine

Vaccine. 2002 May 6;20(15):2007-10. doi: 10.1016/s0264-410x(02)00088-9.

Abstract

Listeria monocytogenes (Lm) is an attractive vector to elicit T cell immunity because it infects antigen-presenting cells and because infection originates at the mucosa. Lm expressing HIV gag elicits sustained high levels of gag-specific CTL in mice. Since Lm causes disease in immunocompromised hosts, a highly attenuated strain of Lm that requires D-Ala for viability was produced. Attenuated bacteria expressing HIV-1 gag (Lmdd-gag) are as efficient as wild-type recombinants at stimulating gag-specific murine CTL when administered with D-Ala and at boosting human CTL in vitro. Lmdd-gag immunization protects mice from vaccinia-gag challenge and induces mucosal CTL, even after systemic immunization.

Publication types

  • Review

MeSH terms

  • AIDS Vaccines* / genetics
  • AIDS Vaccines* / immunology
  • AIDS Vaccines* / toxicity
  • Adult
  • Animals
  • Bacterial Vaccines / immunology
  • Bacterial Vaccines / toxicity
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Female
  • Genes, gag*
  • Genes, nef
  • Genetic Engineering
  • Genetic Vectors / genetics
  • Genetic Vectors / immunology*
  • HIV-1 / genetics
  • HIV-1 / immunology*
  • Humans
  • Immunity, Mucosal
  • Infant, Newborn
  • Listeria monocytogenes / genetics
  • Listeria monocytogenes / immunology*
  • Male
  • Mice
  • Pregnancy
  • Safety
  • T-Lymphocytes, Cytotoxic / immunology
  • Vaccines, Attenuated / immunology
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / immunology
  • Vaccines, Synthetic / toxicity
  • Vaccinia virus / immunology

Substances

  • AIDS Vaccines
  • Bacterial Vaccines
  • Vaccines, Attenuated
  • Vaccines, Synthetic